ABSTRACT
This study explores the use of mobile phones for farm-related activities of ethnic minority farmers in Quang Tri province, Central Vietnam, in the context of the COVID-19 pandemic. A random sampling strategy was used to select 180 ethnic farmers, different by gender, age, and education level, to interview using a semi-structured questionnaire. Results indicate ethnic minority farmers used mobile phones for various purposes related to agriculture through phone calls and social media platforms (Facebook, Zalo, YouTube, etc.). Mobile phones have become essential for farmers to access and exchange market information, receive weather information, get extension advisories, learn new farming practices and technologies, contact and buy farm inputs, etc. There was a statistically significant association between gender, age, and education level with the purposes of mobile phone usage. Young and highly educated farmers should be prioritized in digital service development strategies since they are the pioneers who will be the leading groups of farmers in terms of using mobile phones for farm-related activities. Furthermore, the significance of female farmers' mobile phone use should not be overlooked, as when women have access to these devices, they can use them for various farming tasks to improve their agricultural production. [ABSTRACT FROM AUTHOR] Copyright of Information Services & Use is the property of IOS Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
ABSTRACT
A novel coronavirus associated with acute respiratory disease (named SARS-CoV-2) is recently identified in Wuhan city, China, spread rapidly worldwide. Early identification of this novel coronavirus by molecular tools is critical for surveillance and control of the epidemic outbreak. We aimed to establish a simple method for the detection of SARS-CoV-2 in differentiating with SARS-CoV. Primers of our in-house reverse transcription polymerase chain reaction (RT-PCR) assays were designed to target conserved regions of the RdRP gene and E gene, selected restriction enzymes EcoRI, Tsp45I, and AluI to distinguish between SARS-CoV-2 and SARS-CoV. In this report, a 396-bp fragment of the RdRp gene and 345-bp fragment of the E gene were amplified by one-step RT-PCR. Enzyme Tsp45I cuts the RdRP-amplified product of SARS-CoV-2 generating three fragments of 45, 154, and 197 bp, but it did not cut the amplicon of SARS-CoV. In contrast, the amplified product of SARS-CoV was digested with EcoRI producing two fragments of 76 and 320 bp, whereas the amplicon of SARS-CoV-2 was undigested by Tsp45I help to distinguish clearly SARS-CoV-2 from SARS-CoV on gel electrophoresis. In addition, AluI cut the amplicon of the E gene of SARS-CoV-2 generating two fragments of 248 and 97 bp without cutting to SARS-CoV. The accuracy of the assay was confirmed by sequencing and phylogenetic analysis. When evaluated on clinical samples showed a high sensitivity of 95%, specificity of our assay was 100% and clinical performance for detection of SARS-CoV-2 in comparison with other reference assays. In conclusion, in the present study, we successfully developed a simple method for molecular detection of SARS-CoV-2 in differentiating with SARS-CoV.